{"id":966,"date":"2020-09-15T09:52:33","date_gmt":"2020-09-15T09:52:33","guid":{"rendered":"https:\/\/weisberglab.com\/?page_id=966"},"modified":"2022-09-19T03:12:44","modified_gmt":"2022-09-19T03:12:44","slug":"projects","status":"publish","type":"page","link":"https:\/\/weisberglab.com\/?page_id=966","title":{"rendered":"Research"},"content":{"rendered":"\n<div class=\"wp-block-cover alignfull has-parallax\" style=\"background-image:url(https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/09\/IMG_8054.png);min-height:513px;aspect-ratio:unset;\"><span aria-hidden=\"true\" class=\"wp-block-cover__background has-ast-global-color-2-background-color has-background-dim-0 has-background-dim\"><\/span><div class=\"wp-block-cover__inner-container is-layout-flow wp-block-cover-is-layout-flow\">\n<h1 class=\"has-text-align-center has-white-color has-text-color wp-block-heading\">Research<\/h1>\n<\/div><\/div>\n\n\n\n<div class=\"inherit-container-width wp-block-group alignfull has-ast-global-color-5-background-color has-background is-layout-constrained wp-block-group-is-layout-constrained\"><div class=\"wp-block-group__inner-container\">\n<div class=\"wp-block-columns are-vertically-aligned-center is-layout-flex wp-container-core-columns-is-layout-d0b3c9c8 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Evolution of mobile genetic elements<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img fetchpriority=\"high\" decoding=\"async\" src=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/plasmidnetwork-1024x511.png\" alt=\"\" class=\"wp-image-1426\" width=\"584\" height=\"291\" srcset=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/plasmidnetwork-1024x511.png 1024w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/plasmidnetwork-300x150.png 300w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/plasmidnetwork-768x383.png 768w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/plasmidnetwork.png 1254w\" sizes=\"(max-width: 584px) 100vw, 584px\" \/><figcaption>Characterizing the evolution of mobile genetic elements involved in plant\/microbe interactions and symbioses.<\/figcaption><\/figure>\n\n\n\n<p>Mobile genetic elements (MGEs) play an important role in many plant\/microbe interactions, as well as in human disease, such as those that carry genes conferring antibiotic resistance. MGEs, such as plasmids or integrative conjugative elements (ICEs), can cause bacterial hosts to transition from commensal organisms to pathogens or beneficial mutualists. These selfish genetic elements can recombine as well as transmit horizontally between strains, making understanding their evolution and epidemiology difficult.<br><strong>What are the processes that diversify MGEs? How do they exchange loci with other elements and replicons? How do MGEs evolve and change over time?<\/strong><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Genomic epidemiology<\/h4>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"421\" src=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-1024x421.png\" alt=\"\" class=\"wp-image-1423\" srcset=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-1024x421.png 1024w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-300x123.png 300w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-768x316.png 768w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-1536x632.png 1536w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-2048x843.png 2048w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/visualabstract-1568x645.png 1568w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Population genomics and genomic epidemiology of microbial pathogens and MGEs<\/figcaption><\/figure>\n\n\n\n<p>Our research investigates the genomic epidemiology of diverse agricultural pathogens, including <em>Agrobacterium tumefaciens<\/em> (crown gall disease), <em>Rhodococcus fascians<\/em> (leafy gall disease), <em>Streptomyces scabiei<\/em> (potato common scab), and <em>Xanthomonas hortorum<\/em> pv. <em>carotae<\/em> (disease of carrot), among others.<br>Genomic epidemiology that accurately takes MGEs into account requires generating a phylogenomic framework for accurate whole genome SNP analysis and comparison that minimizes errors and false-positive SNPs. We are developing methods to infer the epidemiology and movement of mobile genetic elements independent of their microbial hosts.<\/p>\n\n\n\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns are-vertically-aligned-center is-layout-flex wp-container-core-columns-is-layout-d0b3c9c8 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"955\" height=\"1024\" src=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa-955x1024.png\" alt=\"\" class=\"wp-image-1424\" srcset=\"https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa-955x1024.png 955w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa-280x300.png 280w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa-768x823.png 768w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa-1433x1536.png 1433w, https:\/\/weisberglab.com\/wp-content\/uploads\/2022\/05\/agromlsa.png 1528w\" sizes=\"(max-width: 955px) 100vw, 955px\" \/><\/figure>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow\">\n<h4 class=\"wp-block-heading\">Determinants of host range and MGE compatibility<\/h4>\n\n\n\n<p>What determines the host range of a pathogen or symbiont? What confers or restricts the host range of a mobile genetic element?<br>Microbial pathogens (and mutualist symbionts) often have a restricted range of host species on which they can cause disease (or provide beneficial services). Some pathogens have a wide host range, while others are more restricted to one or few host species. The genes conferring this phenotype may be present on the chromosome or on mobile genetic elements, or both.<br>Mobile genetic elements may also be restricted to specific taxonomic groups or lineages of microbes in which they can persist. <br><strong>What restricts the host range of a MGE?<\/strong><\/p>\n\n\n\n<p><br><br><br><br><br><br><br><\/p>\n<\/div>\n<\/div>\n<\/div><\/div>\n\n\n\n<div class=\"inherit-container-width wp-block-group alignfull has-ast-global-color-4-background-color has-background is-layout-constrained wp-block-group-is-layout-constrained\"><div class=\"wp-block-group__inner-container\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Evolution of mobile genetic elements Mobile genetic elements (MGEs) play an important role in many plant\/microbe interactions, as well as in human disease, such as those that carry genes conferring antibiotic resistance. MGEs, such as plasmids or integrative conjugative elements (ICEs), can cause bacterial hosts to transition from commensal organisms to pathogens or beneficial mutualists. [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"site-sidebar-layout":"no-sidebar","site-content-layout":"plain-container","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"enabled","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-966","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/pages\/966","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/weisberglab.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=966"}],"version-history":[{"count":26,"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/pages\/966\/revisions"}],"predecessor-version":[{"id":1661,"href":"https:\/\/weisberglab.com\/index.php?rest_route=\/wp\/v2\/pages\/966\/revisions\/1661"}],"wp:attachment":[{"href":"https:\/\/weisberglab.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=966"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}